Small Collections Grant
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Starting DNA and Tissue Collection of SJRP Herbarium
Brazil
Other
Cost (USD):
2000
SJRP
Instituto de Biociências, Letras e Ciências Exatas (IBILCE) – Universidade Estadual Paulista (UNESP)
Objective:
Today, the main demand of the SJRP is basically to increase the collection, especially with specimens from the cerrado and the Amazon region. Furthermore, in line with the global trend in the molecular scope, it is necessary for the SJRP to start a gene bank. Thus, our goals are:
1. Silica gel tissue bank;
2. Total DNA gene bank;
3. Control what is stored in these banks;
4. Increase the Herbarium collection with the material that has been collected;
5. Make the sequences available on GenBank;
6. Provide information and high-resolution images of the exsiccates.
Timetable:
These goals will be achieved through a collection expedition towards the Brazilian Amazon.
Route: State highways to BR-364 -> BR-364 to BR-319 -> BR-319 to Manaus (Amazonas)
Distance: About 7,500 Km
Duration: 20 days
Locations: Parque Nacional das Emas (boundary between Mato Grosso do Sul e Goiás) -> Parque Nacional Mapinguari (Rondônia) -> Parque Nacional Nascentes do Lago Jari (Amazonas) -> Parque Estadual do Matupiri (Amazonas) -> Reserva Florestal Adolpho Ducke (Amazonas); in addition to fragments found on the roadsides.
The Herbarium has the necessary equipment for the collections.
To collect in UCs, we will use our collection license issued by the Sistema de Autorização e Informação em Biodiversidade – SISBIO (Biodiversity Information and Authorization System) of the Ministry of Environment.
Scoring Rubric
Reviewer's name:
Collection Improvement (max. 120 points)
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Facilitating access to the physical collections by digitization (e.g., data entry, setting up database structure with an outline of the platform to be used, purchasing equipment, and imaging specimens) – up to 30 points.
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Enhancing physical collections by improving the conservation status of specimens in the herbarium (e.g., better folders, protecting covers, mounting paper, labeling, etc.) – up to 30 points.
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Curating specimens (e.g., updating families, species identification, identifying types) – up to 20 points.
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Increasing our understanding of the flora or funga by making new herbarium specimens available, such as processing of backlog or collecting and mounting of new specimens from understudied sites – up to 20 points.
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Securing collections by distribution of duplicates (or orphan collections) to other regional or international herbaria or shipping endangered collections to another herbarium – up to 20 points.
This proposal scores:
/120
Methods & Funding (max. 40 points)
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Match between the proposed budget and methods for the aims described – up to 10 points.
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Perceived need, the extent to which the project will benefit from IAPT funding: e.g., due to active floristic work or contribution to poorly collected sites, due to threatened conditions of collections, and for the degree of involvement of others (outreach and education). We give more points for herbaria in low- and middle-income countries – up to 20 points.
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Sharing duplicate specimens with other herbaria – up to 10 points.
This proposal scores:
/40
Broader Impacts (max. 40 points)
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Degree of regional importance of the collection or the taxonomic importance of the targeted collection – up to 10 points.
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The project will yield durable benefits (specimens, digitized metadata, databases, websites) – up to 15 points.
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The project involves outreach/mentoring and broad dissemination – up to 15 points.
This proposal scores:
/40
Year of last successful SCG application:
Has applicant applied for SCG before?:
Plan:
In order to reach our goals, we will take the following actions, so that each number below corresponds to the number of objectives set out in “Your project objective/s”
1. Collect fresh leaf material in the field to store in silica gel;
2. Extract the DNA from the material and store it in a -80º C freezer;
3. Implement an internal database with collected leaf tissue;
4. Collect the branches of plants whose leaves were removed for silica, as is standard procedure for herbarium and taxonomy work;
5. Upload sequences;
6. The SJRP Herbarium is registered with the Centro de Referência em Informação Ambiental (CRIA) and will do this computerization as usual through speciesLink.
It is important to emphasize that representatives of the Dilleniaceae, Elaeocarpaceae, Fabaceae and Pentaphylacaceae families will be collected primarily, as these groups are the focus of research carried out at the SJRP today. But it won't be limited to that.
In Brazil, the accelerated reduction of native vegetation and the consequent loss of biodiversity in the Cerrado and Amazon Domains raises the need to invest in research in the area. Taking advantage of the fact that the SJRP is located in a Cerrado x Atlantic Forest transition region and close to the State of Mato Grosso do Sul, we will carry out collections in fragments of Cerrado in the State of São Paulo and in the neighboring state, prioritizing, but not limited to Units of Conservation (UCs), following a route by car to the Amazon region.
Institution:
IH Code:
Country:
Target areas:
Applicant First Name/s:
email:
Improve herbarium collection and start a gene bank
"Other" target:
Daniela Sampaio Silveira
Applicant Last Name/s: